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\u30d0\u30af\u30c6\u30ea\u30a2\u3092\u5229\u7528\u3057\u305f\u304c\u3093\u6cbb\u7642\u30b7\u30b9\u30c6\u30e0\u3092\u69cb\u7bc9\u3059\u308b\u305f\u3081\u306b\u306f\uff0c\u304c\u3093\u7d44\u7e54\u306b\u30db\u30fc\u30df\u30f3\u30b0\u3059\u308b\u30d0\u30af\u30c6\u30ea\u30a2\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u671b\u307e\u3057\u3044\uff0e\u672c\u7814\u7a76\u3067\u306f\uff0c\u3059\u3067\u306b\u81e8\u5e8a\u306b\u304a\u3044\u3066\u304c\u3093\u6cbb\u7642\u3078\u306e\u5229\u7528\u7d4c\u9a13\u304c\u3042\u308b E. coli Nissle 1917 (EcN)
\u304c\u9078\u629e\u3055\u308c\u305f5)\uff0e\u3053\u306e EcN \u306b\u6e29\u5ea6\u4f9d\u5b58\u6027\u767a\u73fe\u30b9\u30a4\u30c3\u30c1\u3092\u633f\u5165\u3057\uff0c\u6e29\u5ea6\u4e0a\u6607\u3067\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u30b9\u30a4\u30c3\u30c1\u3092\u30aa\u30f3\u306b\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a\u304c\u8a66\u307f\u3089\u308c\u305f\uff0e
\u5b89\u5168\u6027\u3084\u767a\u73fe\u52b9\u7387\u306e\u89b3\u70b9\u304b\u3089\uff0c\u30b9\u30a4\u30c3\u30c1\u30aa\u30d5\u306e\u72b6\u614b\u306b\u304a\u3044\u3066\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u4f4e\u304f\uff0c\u6e29\u5ea6\u4e0a\u6607\u3067\u30b9\u30a4\u30c3\u30c1\u30aa\u30f3\u3068\u306a\u3063\u305f\u72b6\u6cc1\u3067\u6cbb\u7642\u306b\u5fc5\u8981\u306a\u91cf\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u5f97\u3089\u308c\u308b\u30d0\u30af\u30c6\u30ea\u30a2\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u6c42\u3081\u3089\u308c\u308b\uff0e\u305d\u3053\u3067\uff0c\u751f\u4f53\u5185\u3067\u6e29\u5ea6\u5fdc\u7b54\u6027\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\uff0c37\u2103\u4ed8\u8fd1\u3067\u5fae\u5c0f\u306a\u6e29\u5ea6\u5909\u5316\u306b\u53cd\u5fdc\u3059\u308b\u9ad8\u7cbe\u5ea6\u306e\u6e29\u5ea6\u5fdc\u7b54\u6027\u8ee2\u5199\u6291\u5236\u56e0\u5b50\u3092\u5229\u7528\u3057\u3066\uff0c\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u4f5c\u52d5\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u306e\u63a2\u7d22\u304c\u884c\u308f\u308c\u305f\uff0e\u6e29\u5ea6\u5fdc\u7b54\u6027\u8ee2\u5199\u6291\u5236\u56e0\u5b50\u306e\u5019\u88dc\u3068\u3057\u3066\uff0c39\u2103\uff0c38\u2103\u307e\u305f\u306f42\u2103\u306e\u6e29\u5ea6\u95be\u5024\u4ee5\u4e0a\u3067\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b TlpA39\uff0cTcl \u307e\u305f\u306fTcl4
2\u306b\u3064\u3044\u3066\u691c\u8a0e\u3055\u308c
\u305f\uff0eTlpA39 \u306f Salmonella typhimurium \u306b\u5b58\u5728\u3057\uff0c\u52d5\u7269\u306b\u611f\u67d3\u3057\u305f\u969b\u306b\u52d5\u7269\u306e\u4f53\u6e29\u3067\u75c5\u539f\u6027\u907a\u4f1d\u5b50\u7fa4\u306e\u767a\u73fe\u3092\u8abf\u7bc0\u3059\u308b\u56e0\u5b50\u3068\u3057\u3066\u6a5f\u80fd\u3057\u3066\u3044\u308b \uff0e\u307e\u305f\uff0cTcl \u306f\uff0c\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u30e9\u30e0\u30c0\u30bf\u30f3\u30d1
\u30af\u8cea \u201ccl\u201d \u306e\u6e29\u5ea6\u5fdc\u7b54\u6027\u5909\u7570\u4f53\u3067\u3042\u308b\uff0e\u3053\u306e\u201ccl\u201d\u306f\uff0c\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u30e9\u30e0\u30c0\u30a6\u30a4\u30eb\u30b9\u306e\u751f\u4f53\u5185\u3067\u306e\u6f5c\u4f0f\u3092\u6210\u7acb\u3055\u305b\uff0c\u30a6\u30a4\u30eb\u30b9\u306e\u6f5c\u4f0f\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306e\u8ee2\u5199\u6291\u5236\u56e0\u5b50\u3068\u3057\u3066\u6a5f\u80fd\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\uff0e\u3053\u308c\u30893\u7a2e\u985e\u306e\u5019\u88dc\u56e0\u5b50\u306e\u6e29\u5ea6\u5fdc\u7b54\u6027\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\uff0c\u305d\u308c\u305e\u308c\u306e\u767a\u73fe\u6291\u5236\u56e0\u5b50\u3067\u306e\u5236\u5fa1\u4e0b\u3067\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea(GFP)\u3092\u767a\u73fe\u3059\u308b EcN \u3092\u4f5c\u6210\u3057\uff0c37\u2103\u307e\u305f\u306f42\u2103\u3067\u306e\u907a\u4f1d
\u5b50\u767a\u73fe\u304c\u6bd4\u8f03\u3055\u308c\u305f\uff0e\u4eca\u56de\u306e\u6bd4\u8f03\u3067\u306f\uff0c37\u2103\u3067 GFP \u306e\u767a\u73fe\u304c\u307b\u3068\u3093\u3069\u8a8d\u3081\u3089\u308c\u305a\uff0c42\u2103\u3067 GFP \u767a\u73fe\u304c\u5897\u52a0\u3059\u308b\u56e0\u5b50\u3092\u9078\u629e\u3059\u308b\u89b3\u70b9\u3067\u691c\u8a0e\u304c\u884c\u308f\u308c\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c3\u7a2e\u985e\u306e\u8ee2
\u5199\u5236\u5fa1\u56e0\u5b50\u306e\u3046\u3061\uff0cTcl42 \u304c\u4eca\u56de\u306e\u5b9f\u9a13\u76ee\u7684\u3092\u9054\u6210\u3059\u308b\u305f\u3081\u306e\u6a5f\u80fd\u3068\u3057\u3066\u6700\u3082\u512a\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\uff0e\u305d\u3053\u3067\uff0cTcl42 \u3092\u6e29\u5ea6\u5fdc\u7b54\u6027\u767a\u73fe\u8abf\u7bc0\u56e0\u5b50\u3068\u3057\u3066\u9078\u629e\u3055\u308c\u305f\uff0e
\u4eca\u56de\u306e\u691c\u8a0e\u3067\u4f7f\u7528\u3057\u305f\u767a\u73fe\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0\u3067\u306f\uff0c37\u2103\u306b\u304a\u3044\u3066 Tcl42 \u304c\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u4e0a\u6d41\u306e\u8ee2\u5199\u8abf\u7bc0\u90e8\u4f4d\u306b\u7d50\u5408\u3057\uff0c\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u6291\u5236\u3057\u3066\u3044\u308b\uff0e\u305d\u3057\u3066\uff0c42\u2103\u3078\u306e\u52a0\u6e29\u306b\u3088\u308a Tcl42 \u306e\u8ee2\u5199\u6291\u5236\u52b9\u679c\u304c\u306a\u304f\u306a\u308a\uff0c
\u76ee\u7684\u907a\u4f1d\u5b50\u304c\u767a\u73fe\u3059\u308b\u65b9\u5f0f\u304c\u3068\u3089\u308c\u3066\u3044\u308b\uff0e\u3057\u304b\u3057\uff0c\u3053\u306e\u8ee2\u5199\u8abf\u7bc0\u306f\u53ef\u9006\u7684\u3067\u6e29\u5ea6\u4f4e\u4e0b\u306b\u3088\u308a\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u6291\u5236\u3055\u308c\u3066\u3057\u307e\u3046\uff0e\u3053\u306e\u3088\u3046\u306b\u6e29\u5ea6\u4e0a\u6607\u306b\u3088\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u4e00\u904e\u6027\u3067\u3042\u308b\u3068\uff0c\u304c\u3093\u6cbb\u7642\u306b\u304a\u3044\u3066\u5341\u5206\u306a\u6cbb\u7642\u52b9\u679c\u3092\u5f97\u308b\u3053\u3068\u304c\u56f0\u96e3\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u308b\uff0e\u305d\u3053\u3067\uff0c\u6e29\u5ea6\u4e0a\u6607\u3067\u5b89\u5b9a\u306b\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u5f97\u308b\u305f\u3081\u306e\u4ed5\u7d44\u307f\u304c\u52a0\u3048\u3089\u308c\u3066\u3044\u308b\uff0e\u5b9f\u969b\u306b\u306f\uff0cTcl42 \u6291\u5236\u56e0\u5b50\u306b\u3088\u308a\u5236\u5fa1\u3055\u308c\u305f pL\/pR
\u30d5\u30a1\u30fc\u30b8\u30e9\u30e0\u30c0\u6e29\u5ea6\u8a98\u5c0e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u3067\u30bb\u30ea\u30f3\u30fb\u30a4\u30f3\u30c6\u30b0\u30e9\u30fc\u30bc\u3067\u3042\u308b Bxb1
\u3092\u767a\u73fe\u3055\u305b\uff0c attP \u3068 attB \u30b5\u30a4\u30c8\u306b\u631f\u307e\u308c\u305f\u5869\u57fa\u914d\u5217\u7d44\u307f\u66ff\u3048\u3067 T7 \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u30b9\u30a4\u30c3\u30c1\u3092\u30aa\u30f3\u306b\u3057\u3066GFP\u3092\u5b89\u5b9a\u7684\u306b\u767a\u73fe\u3055\u305b\u308b\u30b7\u30b9\u30c6\u30e0\u304c\u69cb\u7bc9\u3055\u308c\u305f\uff0e\u306a\u304a\uff0cBxb1\u306e\u751f\u7406\u7684\u6761\u4ef6\u4e0b\uff08\u672a\u52a0\u6e29\uff09\u3067\u306e\u767a\u73fe\u3092\u5236\u9650\u3059\u308b\u305f\u3081\u306b\uff0cBxb1\u4e0a\u6d41\u306b2\u3064\u306e\u5f37\u529b\u306a\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u304c\u633f\u5165\u3055\u308c\uff0cTcl42\u3067\u306e\u767a\u73fe\u6291\u5236\u6a5f\u80fd\u304c\u5f37\u5316\u3055\u308c\u3066\u3044\u308b\uff0e\u3053\u308c\u306b\u3088\u308a\uff0c\u751f\u7406\u7684\u6761\u4ef6\u4e0b\uff0837\u2103\uff09\u3067\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u4f4e\u304f\uff0c42\u2103\u3078\u306e\u52a0\u6e29\u306b\u3088\u308a\u907a\u4f1d\u5b50\u767a\u73fe\u3092100\u500d\u306b\u5897\u5f37\u3067\u304d\u308b\u30b7\u30b9\u30c6\u30e0\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u3063\u305f\uff0e
\u3000\u305d\u3053\u3067\u6b21\u306b\uff0c\u304c\u3093\u6cbb\u7642\u3078\u306e\u5fdc\u7528\u3092\u8003\u3048\uff0c\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3059\u308bEcN\u306e\u958b\u767a\u304c\u9032\u3081\u3089\u308c\u305f\uff0e\u4eca\u56de\u306f\uff0c\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u3068\u3057\u3066\uff0c\u514d\u75ab\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u963b\u5bb3\u5264\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u6297 CTLA-4 \u30ca\u30ce\u30dc\u30c7\u30a3\u20203\u3092\u767a\u73fe\u3059\u308b EcN
\u306e\u958b\u767a\u304c\u8a66\u307f\u3089\u308c\u305f\uff0e\u5b9f\u969b\u306b\u306f\uff0c\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u3067\u3042\u308b GFP \u767a\u73fe\u306e\u4e0a\u6d41\u306b\u6297
CTLA-4 \u30ca\u30ce\u30dc\u30c7\u30a3\u3092\u767a\u73fe\u3059\u308b\u914d\u5217\u3092\u633f\u5165\u3057\u305f EcN \u304c\u4f5c\u88fd\u3055\u308c\u305f\uff0e\u3053\u306e EcN \u3067\u306e\u6e29\u5ea6\u611f\u53d7\u6027\u306b\u95a2\u3059\u308b\u691c\u8a0e\u304c\u884c\u308f\u308c\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u3053\u306e EcN \u309237\u2103\u30671\u6642\u9593\u57f9\u990a\u3057\u3066\u3082\uff0c\u6297 CTLA-4 \u30ca\u30ce\u30dc\u30c7\u30a3\u306e
\u767a\u73fe\u306f\uff0c\u307b\u3068\u3093\u3069\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\uff0e\u4e00\u65b9\uff0c42\u2103\u307e\u305f\u306f43\u2103\u3067\u57f9\u990a\u3057\u305f\u7d50\u679c\uff0c\u6297
CTLA-4 \u30ca\u30ce\u30dc\u30c7\u30a3\u306e\u9855\u8457\u306a\u767a\u73fe\u304c\u8a8d\u3081\u3089\u308c\u305f\uff0e\u307e\u305f\uff0c42\u2103\u3088\u308a43\u2103\u306e\u65b9\u304c\uff0c\u6297
CTLA-4 \u30ca\u30ce\u30dc\u30c7\u30a3\u306e\u767a\u73fe\u91cf\u304c\u9ad8\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\uff0e\u3053\u306e\u3088\u3046\u306b\uff0c\u6cbb\u7642\u7528\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u52a0\u6e29\u306b\u3088\u308a\u5236\u5fa1\u3067\u304d\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\uff0e\u305d\u3053\u3067\uff0c\u3053\u306e EcN \u3092\u7528\u3044\u3066\u62c5\u304c\u3093\u30de\u30a6\u30b9\u3067\u306e\u6cbb\u7642\u5b9f\u9a13\u304c\u884c\u308f\u308c
\u305f\uff0eBALB\/c \u30de\u30a6\u30b9\u306e\u80cc\u90e8\u76ae\u5185\u306bB\u7d30\u80de\u30ea\u30f3\u30d1\u816b\u7d30\u80de\u682a\uff08A20\uff09\u3092\u79fb\u690d\u3057\uff0c\u79fb\u690d7\u65e5\u5f8c\uff08\u816b\u760d\u4f53\u7a4d\u304c\u7d04 100 mm3 \uff09\u306b\uff0c\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3057\u306a\u3044(Wild type) EcN \u307e\u305f\u306f\u6cbb\u7642\u7528
\u907a\u4f1d\u5b50\u767a\u73fe EcN \u3092\u5c3e\u9759\u8108\u304b\u3089\u6295\u4e0e\u3057\u305f\uff0e\u306a\u304a\uff0c\u4eca\u56de\u306e\u691c\u8a0e\u3067\u306f\uff0c\u6297CTLA-4\u30ca\u30ce\u30dc\u30c7\u30a3\u767a\u73fe EcN \u3068\u6297PD-1\u30ca\u30ce\u30dc\u30c7\u30a3\u767a\u73fe EcN \u30921\uff1a1\u3067\u6df7\u5408\u3057\u305f EcN \u304c\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u767a\u73fe
EcN \u3068\u3057\u3066\u6295\u4e0e\u3055\u308c\u305f\uff0eEcN \u6295\u4e0e2\u65e5\u5f8c\u306b\uff0c\u304c\u3093\u7d44\u7e54\u306b\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u7167\u5c04\u3057\uff0c\u304c\u3093\u7d44\u7e54\u309243\u2103\u306b\u52a0\u6e29\u3057\u305f\uff0e\u52a0\u6e29\u6761\u4ef6\u306f\uff0c\u8d85\u97f3\u6ce2\u30aa\u30f35\u5206\u9593\u306743\u2103\u306b\u52a0\u6e29\uff0c\u8d85\u97f3\u6ce2\u30aa\u30d55\u5206\u9593\u3067\u751f\u7406\u7684\u6e29\u5ea6\uff0837\u2103\uff09\u306e\u30b5\u30a4\u30af\u30eb\u30926\u56de\u7e70\u308a\u8fd4\u3057\u305f\uff08\u6cbb\u7642\u6642\u9593\u3068\u3057\u3066\u306f60\u5206\u9593\uff08\u30c8\u30fc\u30bf\u30eb\u52a0\u6e29\u6642\u959330\u5206\uff09\uff09\uff0e\u305d\u306e\u5f8c\uff0c\u816b\u760d\u306e\u4f53\u7a4d\u5909\u5316\u3092\u6307\u6a19\u306b\u6297\u816b\u760d\u52b9\u679c\u3092\u8a55\u4fa1\u3057\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0cWild type \u306e EcN \u6295\u4e0e\u7fa4\u304a\u3088\u3073\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u767a
\u73fe EcN \u3092\u6295\u4e0e\u3057\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u7167\u5c04\u3057\u306a\u304b\u3063\u305f\u7fa4\u3067\u306f\uff0c\u816b\u760d\u306e\u5897\u6b96\u6291\u5236\u52b9\u679c\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\uff0e\u4e00\u65b9\uff0c\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u767a\u73fe EcN \u3092\u6295\u4e0e\u3057\uff0c\u96c6\u675f\u8d85\u97f3\u6ce2\u3067\u306e\u52a0\u6e29\u3092\u884c\u3063\u305f\u7fa4\u306b\u304a\u3044\u3066\u9855\u8457
\u306a\u816b\u760d\u306e\u5897\u6b96\u6291\u5236\u52b9\u679c\u304c\u8a8d\u3081\u3089\u308c\u305f\uff0e\u305d\u3053\u3067\uff0c\u6295\u4e0e\u3057\u305f EcN \u306e\u816b\u760d\u5185\u3067\u306e\u6d3b\u6027\u5316\u306b\u3064\u3044\u3066\u691c\u8a0e\u3057\u305f\u3068\u3053\u308d\uff0c\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u7167\u5c04\u3057\u305f\u7fa4\u3067 EcN \u304c\u6d3b\u6027\u5316\u3055\u308c\u3066\u304a\u308a\uff0c\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u7167\u5c04\u3057\u306a\u3044\u7fa4\u3067
\u306f\uff0cEcN \u306e\u6d3b\u6027\u5316\u304c\u307b\u3068\u3093\u3069\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\uff0e\u307e\u305f\uff0c\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u304c\u3093\u7d44\u7e54\u306b\u7167\u5c04\u3057\u305f\u30de\u30a6\u30b9\u306b\u304a\u3044\u3066\uff0c\u6d3b\u6027\u5316\u3057\u305f EcN \u306f\uff0c\u4e3b\u306b\u816b\u760d\u5185\u3067\u8a8d\u3081\u3089\u308c\uff0c\u809d\u81d3\u3084\u813e\u81d3\u3067\u306f\u307b\u3068\u3093\u3069\u8a8d\u3081\u3089
\u308c\u306a\u304b\u3063\u305f\uff0e\u3053\u306e\u3053\u3068\u304b\u3089\uff0c\u6e29\u5ea6\u5fdc\u7b54\u6027 EcN \u3068\u96c6\u675f\u8d85\u97f3\u6ce2\u3092\u5229\u7528\u3057\u305f\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8abf\u7bc0\u6cd5\u306f\uff0c\u304c\u3093\u7d44\u7e54\u7279\u7570\u7684\u306a\u6cbb\u7642\u7528\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u8a98\u5c0e\u3067\u304d\u308b\u526f\u4f5c\u7528\u306e\u5c11\u306a\u3044\u65b0\u305f\u306a\u304c\u3093\u6cbb\u7642\u6226\u7565\u306b\u306a\u308b\u3082\u306e\u3068\u671f\u5f85\u3055\u308c\u308b\uff0e\u306a\u304a\uff0c\u672c\u7a3f\u3067\u7d39\u4ecb\u3057\u305f\u8ad6\u6587\u4ee5\u5916\u306b\u3082\uff0c\u30d0\u30af\u30c6\u30ea\u30a2\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u96c6\u675f\u8d85\u97f3\u6ce2\u306b\u3088\u308b\u52a0\u6e29\u3067\u8abf\u7bc0\u3057\uff0c\u304c\u3093\u514d\u75ab\u7642\u6cd5\u3092\u884c\u3046\u65b9\u6cd5\u304c\u5225\u306e\u30b0\u30eb\u30fc\u30d7\u304b\u3089 Nat. commun. \u306b\u5831\u544a6)\u3055\u308c\u3066\u304a
\u308a\uff0c\u672c\u6280\u8853\u306e\u4eca\u5f8c\u306e\u7814\u7a76\u52d5\u5411\u304c\u6c17\u306b\u306a\u308b\u3068\u3053\u308d\u3067\u3042\u308b\uff0e
\u53c2\u8003\u6587\u732e
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tumor immunotherapy. Nat Commun, 13: 4468, 2022.
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Engineering of Probiotic Bacteria System for the Temperature-sensitive
Production of Immune Checkpoint Blockade Nanobodies by Intratumor Heating
with Focused Ultrasound
RYO SUZUKI*
Faculty of Pharma-Science, Advanced Comprehensive Research Organization
(ACRO), Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan
*corresponding author: r-suzuki@pharm.teikyo-u.ac.jp<\/a>
Keywords: Probiotics, Focused ultrasound, Cancer immunotherapy, Checkpoint
blockage, Nanobody
Received: 9 September, 2022
Accepted: 17 November, 2022
Thermal Medicine\u3000 Vol.38(4)\u63b2\u8f09\u4e88\u5b9a<\/p>\n","protected":false},"excerpt":{"rendered":"