\u30cf\u30a4\u30d1\u30fc\u30b5\u30fc\u30df\u30a2\u306b\u95a2\u3059\u308b\u6700\u8fd1\u306e\u8a71\u984c29<\/p>\n\n\n\n
Hsp70\u3092\u6838\u306b\u8f38\u9001\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8ceaHikeshi (\u706b\u6d88\u3057)\u306e\u5f79\u5272\u306b\u3064\u3044\u3066<\/p>\n\n\n\n
\u67da\u6728 \u9054\u4e5f\uff0c\u7530\u6e15 \u572d\u7ae0 (\u5bcc\u5c71\u5927\u5b66)<\/p>\n\n\n\n
\u30cf\u30a4\u30d1\u30fc\u30b5\u30fc\u30df\u30a2 (HT)\u3084\u71b1\u306e\u7814\u7a76\u9818\u57df\u306b\u304a\u3044\u3066\uff0c\u71b1\u30b7\u30e7\u30c3\u30af\u30bf\u30f3\u30d1\u30af\u8ceaHsp70\u306f\u6700\u3082\u826f\u304f\u7814\u7a76\u3055\u308c\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e00\u3064\u3067\u3042\u308b\uff0e\u7d30\u80de\u304c\u71b1\u30b9\u30c8\u30ec\u30b9\u7b49\u306e\u30b9\u30c8\u30ec\u30b9\u306b\u66b4\u9732\u3055\u308c\u305f\u6642\uff0cHsp70\u306f\u901f\u3084\u304b\u306b\u7d30\u80de\u8cea\u304b\u3089\u6838\u3078\u8f38\u9001\u3055\u308c\u308b\uff0e2012\u5e74\uff0c\u3053\u306e\u8f38\u9001\u306b\uff0cImportin \u30d5\u30a1\u30df
\u30ea\u30fc\u306b\u5c5e\u3055\u306a\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6a5f\u80fd\u304c\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u3044\u306a\u304b\u3063\u305f C11orf73\u907a\u4f1d\u5b50\u7523\u7269\u304c\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u308a\uff0cHikeshi (\u706b\u6d88\u3057)\u3068\u547d\u540d\u3055\u308c\u305f1)\uff0e\u3053\u306e\u767a\u898b\u4ee5\u964d\uff0c\u71b1\u30b9\u30c8\u30ec\u30b9\u306b\u95a2\u9023\u3059\u308b\u7814
\u7a76\u3092\u884c\u3063\u3066\u3044\u308b\u7b46\u8005\u306f\uff0c\u5e38\u306bHikeshi\u306e\u7814\u7a76\u52d5\u5411\u306b\u6ce8\u76ee\u3057\u3066\u304d\u305f\uff0e\u6700\u8fd1\u306eHikeshi\u306b\u95a2\u9023\u3059\u308b\u6210\u679c\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3059\u308b\uff0e
\u81e8\u5e8a\u80c3\u304c\u3093\u691c\u4f53\u306b\u304a\u3044\u3066\uff0cHikeshi\u306f\u304c\u3093\u7d44\u7e54\u3067\u767a\u73fe\u30ec\u30d9\u30eb\u304c\u9ad8\u304f\uff0c\u305d\u306e\u767a\u73fe\u306f\u30ea\u30f3\u30d1\u7ba1\u4fb5\u8972\u3068\u6709\u610f\u306b\u76f8\u95a2\u3057\u305f\uff0e\u4e8c\u7a2e\u985e\u306e\u30d2\u30c8\u80c3\u304c\u3093\u7d30\u80de\u682a\u3092\u7528\u3044\u305f\u691c\u8a0e\u306b\u304a\u3044\u3066\uff0c\u975e\u30b9\u30c8\u30ec\u30b9\u8ca0\u8377\u6761\u4ef6\u3067\u306f\uff0cHikeshi\u306e\u30ce\u30c3\u30af\u30c0\u30a6\u30f3 (KD)\u306f\u7d30\u80de\u306e\u751f\u5b58\u7387\u306b
\u5f71\u97ff\u3092\u4e0e\u3048\u306a\u304b\u3063\u305f\uff0e\u4e00\u65b9\uff0c43 oC\uff0c3\u6642\u9593\u306e\u6e29\u71b1\u30b9\u30c8\u30ec\u30b9\u8ca0\u8377\u306e24\u6642\u5f8c\u3067\u306f\uff0cHikeshi\u306eKD\u306f\u7d30\u80de\u751f\u5b58\u7387\u3092\u6709\u610f\u306b\u4f4e\u4e0b\u3055\u305b\uff0c\u3053\u306e\u6761\u4ef6\u4e0b\u3067\u306f\uff0cHsp70\u306e\u6838\u5185\u79fb\u884c\u304c\u6291\u5236\u3055\u308c\u3066\u3044\u305f\uff0e\u4ee5\u4e0a\u3088\u308a\uff0cHikeshi\u306fHT\u306e\u65b0\u898f\u306e\u6a19\u7684\u907a\u4f1d\u5b50\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b2)\uff0e
\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u7528\u3044\u3066\uff0c\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093\u7d30\u80deHeLa\u3068\u30c6\u30ed\u30e1\u30a2\u9006\u8ee2\u5199\u9175\u7d20\u3067\u4e0d\u6b7b\u5316\u3055\u308c\u305f\u30d2\u30c8\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de\u682ahTERT-RPE1 (RPE)\u304b\u3089\u5404\u3005\u306eHikeshi\u30ce\u30c3\u30af\u30a2\u30a6\u30c8 (KO)\u7d30\u80de\u304c\u69cb\u7bc9\u3055\u308c\u305f\uff0e\u6e29\u71b1\u30b9
\u30c8\u30ec\u30b9 (43 oC\uff0c1\u6642\u9593)\u3084\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u963b\u5bb3\u5264\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u6bd2\u6027\u304c\u8ca0\u8377\u3055\u308c\u305f\u6761\u4ef6\u3067\u306f\uff0cHeLa Hikeshi KO\u7d30\u80de\u306f\u89aa\u7d30\u80de\u306b\u6bd4\u3079\u3066\u6709\u610f\u306b\u7d30\u80de\u5897\u6b96\u304c\u4f4e\u4e0b\u3057\uff0c\u3053\u308c\u306b\u306fHsp70
\u306e\u6838\u79fb\u884c\u6291\u5236\u306b\u3088\u308b\u6838\u5185\u306b\u304a\u3051\u308bHSF1\u306e\u6a5f\u80fd\u969c\u5bb3\u304c\u95a2\u9023\u3057\u3066\u3044\u308b\u3068\u7d50\u8ad6\u3055\u308c\u305f\uff0e\u4e00\u65b9\uff0cRPE Hikeshi KO\u7d30\u80de\u306f\u89aa\u7d30\u80de\u306b\u6bd4\u3079\u3066\u7d30\u80de\u4fdd\u8b77\u7684\u306a\u4f5c\u7528\u3092\u8a8d\u3081\uff0c\u30b9\u30c8\u30ec\u30b9\u62b5\u6297\u6027\u306e\u6027
\u8cea\u3092\u793a\u3057\u305f\uff0e\u3053\u308c\u306b\u306f\uff0cp53\u3068\u305d\u306e\u4e0b\u6d41\u306b\u3042\u308bp21\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306e\u4e0a\u6607\u304c\u95a2\u4e0e\u3059\u308b\u3068\u7d50\u8ad6\u3055\u308c\u305f3)\uff0e\u4eca\u5f8c\u3001Hikeshi\u3068p53-p21\u3068\u306e\u95a2\u4fc2\u306e\u8a73\u7d30\u304c\u89e3\u660e\u3055\u308c\u308b\u3053\u3068\u3092\u671f\u5f85\u3057\u3066\u3044\u308b\uff0e
\u30d2\u30c8\u306eHikeshi\u907a\u4f1d\u5b50\u5909\u7570\u3068\u75be\u60a3\u3068\u306e\u95a2\u9023\u6027\u3092\u793a\u3057\u305f\u8208\u5473\u6df1\u3044\u5831\u544a\u304c\u3042\u308b\uff0e\u30a2\u30b7\u30e5\u30b1\u30ca\u30fc\u30b8\u7cfb\u30e6\u30c0\u30e4\u4eba\u60a3\u8005\u306b\u304a\u3044\u3066\uff0cHikeshi\u306eVal54Leu\u306e\u30db\u30e2\u63a5\u5408\u578b\u70b9\u5909\u7570\u306f\uff0c\u767d\u8cea\u8133\u75c7\u3084\u65e9\u6b7b\u3092\u8a98\u5c0e\u3057\u305f4)\uff0e\u307e\u305f\uff0cHikeshi\u306eCys4Ser\u306e\u30db\u30e2\u63a5\u5408\u578b\u70b9\u5909\u7570\u306f\uff0c\u30d5\u30a3\u30f3\u30e9\u30f3\u30c9\u4eba\u5c0f\u5150\u306e\u30df\u30a8\u30ea\u30f3\u5f62\u6210\u6e1b\u5c11\u3092\u4f34\u3046\u767d\u8cea\u8133\u75c7\u306b\u95a2\u9023\u3059\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u305f5)\uff0eHikeshi\u306e\u7acb\u4f53\u69cb\u9020\u3092\u57fa\u306b\u3057\u3066\uff0c\u524d\u8005\u306e\u5909\u7570\u306fHikeshi\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e0d\u5b89\u5b9a\u53164)\uff0c\u5f8c\u8005\u306fHikeshi\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30db\u30e2\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u963b\u5bb35)\u304c\u539f\u56e0\u3067Hikeshi\u306e\u6a5f\u80fd\u969c\u5bb3\u306b\u7e4b\u304c\u3063\u305f\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\uff0e
HT\u306b\u304a\u3051\u308bHikeshi\u306e\u7814\u7a76\u3092\u542b\u3081\uff0c\u4eca\u5f8c\u306e\u672c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7814\u7a76\u306e\u9032\u5c55\u306b\u671f\u5f85\u3057\u305f\u3044\uff0e<\/p>\n\n\n\n
\u53c2\u8003\u6587\u732e
1) Kose S., et al. Hikeshi, a nuclear import carrier for Hsp70s, protects
cells from heat shock-induced nuclear damage. Cell, 149: 578-89, 2012.
2) Yanoma T., et al. Heat shock-induced HIKESHI protects cell viability via
nuclear translocation of heat shock protein 70. Oncol Rep, 38: 1500-6, 2017.
3) Rahman K.M.Z., et al. Hikeshi modulates the proteotoxic stress response
in human cells: Implication for the importance of the nuclear function of
HSP70s. Genes Cells, 22: 968-76, 2017.
4) Edvardson S., et al. Leukoencephalopathy and early death associated with
an Ashkenazi-Jewish founder mutation in the Hikeshi gene. J Med Genet, 53:
132-7, 2016.
5) Vasilescu C., et al. Absence of Hikeshi, a nuclear transporter for
heat-shock protein HSP70, causes infantile hypomyelinating
leukoencephalopathy. Eur J Hum Genet, 25: 366-70, 2017.<\/p>\n","protected":false},"excerpt":{"rendered":"
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